6L25

Deoxyribonuclease from Staphylococcus aureus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97930
Spacegroup name	P 1 21 1
Unit cell lengths	47.627, 77.832, 77.378
Unit cell angles	90.00, 98.81, 90.00

Refinement procedure

Resolution	30.000 - 1.850
R-factor	0.2118
Rwork	0.210
R-free	0.23560
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.769
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.880
High resolution limit [Å]	1.850	1.850
Number of reflections	47022	2125
<I/σ(I)>	41.9
Completeness [%]	98.6	89.4
Redundancy	9.6	7.8
CC(1/2)	0.989	0.872

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2.4 M sodium malonate, pH 7.0