6L1Y
structure of gp120/CD4 with a non-canonical surface
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.444, 66.468, 73.850 |
| Unit cell angles | 90.00, 108.40, 90.00 |
Refinement procedure
| Resolution | 33.815 - 2.469 |
| R-factor | 0.2061 |
| Rwork | 0.203 |
| R-free | 0.25680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tgt |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.342 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL2Map |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.469 | 2.470 |
| Rmerge | 0.050 | 0.470 |
| Number of reflections | 21690 | 1803 |
| <I/σ(I)> | 21.6 | 2.15 |
| Completeness [%] | 95.6 | 96.8 |
| Redundancy | 3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.4 | 293 | 0.1 M HEPES sodium, pH 7.4, 11% PEG 4000 and 10% 2-propanol |
