6L1M

Structure of human StAR-related lipid transfer protein 4 mutant - LWNI107-110GG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-23
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	42.914, 43.357, 89.798
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.020 - 1.700
R-factor	0.1686
Rwork	0.168
R-free	0.19010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6l1d
RMSD bond length	0.005
RMSD bond angle	0.794
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.088	0.432
Number of reflections	19187	943
<I/σ(I)>	33.8	5.5
Completeness [%]	99.3
Redundancy	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	287	0.1M MES pH 6.0, 25% PEG 3350, 200 mM NaCl