6L1E
Crystal structure of middle domain of hSSRP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-19 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.002, 66.210, 84.501 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.300 - 2.094 |
R-factor | 0.1878 |
Rwork | 0.186 |
R-free | 0.22450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gcj |
RMSD bond length | 0.007 |
RMSD bond angle | 0.865 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.300 | 2.180 |
High resolution limit [Å] | 2.094 | 2.094 |
Rmerge | 0.087 | 0.376 |
Number of reflections | 13367 | 1270 |
<I/σ(I)> | 26.94 | |
Completeness [%] | 99.1 | |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 10mM MES pH4.0, 12.5% 2-propanol, 20%(w/v) PEG 2000 |