6L1D
Structure of human StAR-related lipid transfer protein 4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-01 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97950 |
Spacegroup name | P 41 |
Unit cell lengths | 93.770, 93.770, 73.275 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.750 - 1.950 |
R-factor | 0.1892 |
Rwork | 0.188 |
R-free | 0.21650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jss |
RMSD bond length | 0.006 |
RMSD bond angle | 0.759 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.073 | 0.440 |
Number of reflections | 45852 | 2304 |
<I/σ(I)> | 32.5 | 4.2 |
Completeness [%] | 98.7 | |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 287 | 0.1 M Bicine pH 9.0, 30% PEG 1000 |