6L1C
Crystal Structure Of of PHF20L1 Tudor1 Y24L mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-03-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 41 |
| Unit cell lengths | 52.570, 52.570, 38.646 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.170 - 1.580 |
| R-factor | 0.1792 |
| Rwork | 0.178 |
| R-free | 0.19090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sd4 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.161 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.173 | 1.623 |
| High resolution limit [Å] | 1.580 | 1.582 |
| Rmerge | 0.097 | 0.341 |
| Number of reflections | 13228 | 956 |
| <I/σ(I)> | 37.4 | 6.3 |
| Completeness [%] | 99.9 | |
| Redundancy | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.6M lithium sulfate, 0.1M Tris, PH 8.0 |
