6L0Y

Structure of dsRNA with G-U wobble base pairs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-05-19
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	H 3 2
Unit cell lengths	45.080, 45.080, 354.130
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.610 - 3.000
R-factor	0.2374
Rwork	0.232
R-free	0.27910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	455d
RMSD bond length	0.002
RMSD bond angle	0.412
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.610	3.107
High resolution limit [Å]	3.000	3.000
Rmerge	0.043	0.099
Rmeas	0.047	0.108
Rpim	0.018	0.042
Number of reflections	3067	280
<I/σ(I)>	30.62	12.26
Completeness [%]	98.7	99.29
Redundancy	6.4	6.7
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30% PEG 4000, 0.2M Ammonium Sulfate