6L0X

The First Tudor Domain of PHF20L1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-05-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	25.346, 32.019, 47.350
Unit cell angles	84.99, 88.22, 82.84

Refinement procedure

Resolution	25.317 - 1.300
R-factor	0.154
Rwork	0.152
R-free	0.18180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sd4
RMSD bond length	0.005
RMSD bond angle	0.928
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.320	1.350
High resolution limit [Å]	1.300	1.300
Rmerge	0.049	0.363
Number of reflections	29808	1817
<I/σ(I)>	21.2
Completeness [%]	89.3
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	10% PEG 6000, 0.1M citric acid, PH 4.0