6L0O
WH domain of human MCM8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 61 |
| Unit cell lengths | 52.062, 52.062, 50.468 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.620 - 1.210 |
| R-factor | 0.1225 |
| Rwork | 0.120 |
| R-free | 0.14650 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.856 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | Sir2014 |
| Refinement software | PHENIX (dev_3139) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.230 |
| High resolution limit [Å] | 1.210 | 1.210 |
| Rmerge | 0.064 | 0.633 |
| Rmeas | 0.065 | 0.649 |
| Rpim | 0.014 | 0.144 |
| Number of reflections | 23189 | 1100 |
| <I/σ(I)> | 51.576 | 5.714 |
| Completeness [%] | 97.3 | 93 |
| Redundancy | 20.3 | 19.8 |
| CC(1/2) | 0.997 | 0.932 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.0-2.2M (NH4)2SO4, 0.1M Tris-HCl pH 8.0 |
