6L0K

Crystal structure of dihydroorotase in complex with malate at pH9 from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-17
Detector	RAYONIX MX300HE
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	85.824, 88.856, 103.751
Unit cell angles	90.00, 95.38, 90.00

Refinement procedure

Resolution	46.156 - 3.300
R-factor	0.2168
Rwork	0.214
R-free	0.26560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6l0a
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.156	3.420
High resolution limit [Å]	3.300	3.300
Number of reflections	22773	1822
<I/σ(I)>	17.7
Completeness [%]	96.4
Redundancy	3.7
CC(1/2)	0.931	0.740

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	291	100mM MMT/sodium hydroxide pH 9, 26% PEG 1500