6L0B
Crystal structure of dihydroorotase in complex with fluorouracil from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-16 |
| Detector | RAYONIX MX325HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.474, 88.430, 103.165 |
| Unit cell angles | 90.00, 95.41, 90.00 |
Refinement procedure
| Resolution | 29.940 - 2.700 |
| R-factor | 0.193 |
| Rwork | 0.190 |
| R-free | 0.24860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6l0a |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.940 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 41606 | 3807 |
| <I/σ(I)> | 9.9 | |
| Completeness [%] | 98.4 | |
| Redundancy | 4.1 | |
| CC(1/2) | 0.934 | 0.744 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 10 mM imidazole malate pH 7.0, 19 % PEG 4000 |
