6L0B

Crystal structure of dihydroorotase in complex with fluorouracil from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-16
Detector	RAYONIX MX325HE
Wavelength(s)	0.9
Spacegroup name	P 1 21 1
Unit cell lengths	85.474, 88.430, 103.165
Unit cell angles	90.00, 95.41, 90.00

Refinement procedure

Resolution	29.940 - 2.700
R-factor	0.193
Rwork	0.190
R-free	0.24860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6l0a
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.940	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	41606	3807
<I/σ(I)>	9.9
Completeness [%]	98.4
Redundancy	4.1
CC(1/2)	0.934	0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	10 mM imidazole malate pH 7.0, 19 % PEG 4000