6KYS

The structure of the M. tb toxin MazF-mt1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Collection date	2019-05-07
Detector	OXFORD ONYX CCD
Wavelength(s)	1.54
Spacegroup name	P 21 21 2
Unit cell lengths	52.140, 72.485, 60.975
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.460 - 2.200
R-factor	0.212967304022
Rwork	0.211
R-free	0.25863
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hjz
RMSD bond length	0.005
RMSD bond angle	0.773
Data reduction software	CrysalisPro
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.460	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.250	0.630
Number of reflections	12032	1728
<I/σ(I)>	5.8	2.2
Completeness [%]	98.7	99.3
Redundancy	4.8
CC(1/2)	0.966	0.669

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	20% PEG 3350, 0.1 M NaOAc pH 5.0, and 0.1 M NaCl