6KXA

Galectin-3 CRD binds to GalA dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	35.876, 57.276, 61.867
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.403 - 1.230
R-factor	0.1674
Rwork	0.166
R-free	0.18450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.011
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.403	1.250
High resolution limit [Å]	1.230	1.230
Rmerge	0.047	0.628
Number of reflections	37730	1853
<I/σ(I)>	18.1
Completeness [%]	99.8
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG