6KX2

Crystal structure of GDP bound RhoA protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	91.819, 91.819, 56.861
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.342 - 1.454
R-factor	0.1855
Rwork	0.184
R-free	0.21040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ftn
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.342	1.506
High resolution limit [Å]	1.454	1.454
Rmerge	0.113	0.636
Number of reflections	43269	4263
<I/σ(I)>	22.97
Completeness [%]	100.0
Redundancy	26
CC(1/2)	0.997	0.944

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	289	10 mg/mL mutant, 0.2M NaCl; 0.1M acetate Na (pH 4.5) and 1.26M ammonium sulfate