6KWQ

Crystal structure of enterovirus 71 polymerase elongation complex (native form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-19
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	124.301, 75.544, 74.031
Unit cell angles	90.00, 91.46, 90.00

Refinement procedure

Resolution	47.001 - 1.760
R-factor	0.1824
Rwork	0.181
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5f8g
RMSD bond length	0.006
RMSD bond angle	0.827
Data reduction software	d*TREK
Data scaling software	d*TREK (9.9.9.4D)
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.001	47.001	1.820
High resolution limit [Å]	1.760	3.790	1.760
Rmerge	0.077	0.044	0.426
Rmeas	0.090	0.052	0.498
Number of reflections	67766	6890	6740
<I/σ(I)>	7.8	22.3	2
Completeness [%]	99.8	99.5	99.9
Redundancy	3.76	3.71	3.74

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	289	PEG 4000