6KWO
Crystal structure of pSLA-1*1301 complex with mutant epitope ESDTVGWSW
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97931 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 218.570, 45.780, 45.450 |
Unit cell angles | 90.00, 99.28, 90.00 |
Refinement procedure
Resolution | 53.928 - 1.803 |
R-factor | 0.2049 |
Rwork | 0.204 |
R-free | 0.22310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qq3 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.765 |
Data reduction software | HKL-3000 ((1.14_3247: ???)) |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.14_3247: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.928 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.117 | 0.734 |
Number of reflections | 38597 | 38597 |
<I/σ(I)> | 9.9 | 2.2 |
Completeness [%] | 93.8 | |
Redundancy | 7.1 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.15 M Potassium Bromide, 30% w/v Polyethylene Glycol Monomethyl ether 2000 |