6KVX

Crystal structure of the N-terminal domain single mutant (D119A) of the human mitochondrial calcium uniporter fused with T4 lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 65
Unit cell lengths	97.899, 97.899, 61.619
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.960 - 2.850
R-factor	0.18357
Rwork	0.179
R-free	0.26218
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2lzm 4xtb
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.850	2.850
Rmerge	0.093	0.507
Number of reflections	7820	413
<I/σ(I)>	12.3
Completeness [%]	98.7
Redundancy	4.2
CC(1/2)	0.943	0.708

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% (w/v) polyethylene glycol (PEG) 3350, 5% (v/v) glycerol, 0.3 M (NH4)2SO4, and 0.1 M Bis-Tris-HCl (pH 5.5)