6KVM
Crystal structure of Chicken MHC Class II for 1.9 angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-20 |
| Detector | Nonius Kappa CCD |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.397, 57.297, 58.057 |
| Unit cell angles | 90.00, 110.68, 90.00 |
Refinement procedure
| Resolution | 54.377 - 1.897 |
| Rwork | 0.203 |
| R-free | 0.22740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aqd |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.290 | 1.950 |
| High resolution limit [Å] | 1.897 | 1.900 |
| Rmerge | 0.097 | |
| Rmeas | 0.113 | |
| Rpim | 0.042 | 0.274 |
| Number of reflections | 37303 | |
| <I/σ(I)> | 15.1 | 3.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.4 | 7.1 |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 30% PEG 4000, 100mM Tris base/Hydrochloric acid pH 8.5, 200mM Lithium sulfate |
