6KVK

Crystal structure of UDP-Sm-SrUGT76G1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9798
Spacegroup name	P 31 2 1
Unit cell lengths	98.013, 98.013, 89.529
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.051 - 2.397
R-factor	0.2062
Rwork	0.204
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vce
RMSD bond length	0.021
RMSD bond angle	1.873
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.051	2.483
High resolution limit [Å]	2.397	2.397
Rmeas	0.142
Number of reflections	19809	1864
<I/σ(I)>	22.5
Completeness [%]	100.0
Redundancy	19.2
CC(1/2)	0.986	0.803

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	24% (vol/vol) 2-propanol, 0.1 M sodium citrate tribasic dihydrate, pH 5.9, 20% (wt/vol) PEG4000