6KVI

Crystal structure of UDP-SrUGT76G1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9798
Spacegroup name	P 31 2 1
Unit cell lengths	98.058, 98.058, 91.618
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.228 - 2.598
R-factor	0.1941
Rwork	0.191
R-free	0.24890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vce
RMSD bond length	0.012
RMSD bond angle	1.982
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.228	2.691
High resolution limit [Å]	2.598	2.598
Rmeas	0.147
Number of reflections	15678	1344
<I/σ(I)>	12.2
Completeness [%]	99.3
Redundancy	6
CC(1/2)		0.785

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	18% (vol/vol) 2-propanol, 0.1 M sodium citrate tribasic dihydrate, pH 5.5, 20% (wt/vol) pEG4000