Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-23 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 92.663, 194.443, 74.384 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.940 - 2.790 |
| R-factor | 0.2238 |
| Rwork | 0.221 |
| R-free | 0.27710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e6j 1fdl |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.488 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.850 |
| High resolution limit [Å] | 2.750 | 5.910 | 2.750 |
| Rmerge | 0.171 | 0.068 | |
| Total number of observations | 131713 | ||
| Number of reflections | 17815 | 1861 | 1745 |
| <I/σ(I)> | 4.5 | ||
| Completeness [%] | 99.7 | 98.1 | 100 |
| Redundancy | 7.4 | 7 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 8 | 293 | PEG 400, magnesium sulphate, bicine |
