6KRB
High resolution crystal structure of an Acylphosphatase protein cage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-07-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 |
| Unit cell lengths | 104.310, 104.310, 146.430 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.132 - 2.375 |
| R-factor | 0.196 |
| Rwork | 0.194 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hi2 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.504 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (phenix.refine: 1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.900 | 2.450 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.149 | |
| Rpim | 0.505 | |
| Number of reflections | 71399 | 3134 |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 97.8 | |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 2.4M AMS, 0.1M Citric acid pH 4.0 |
