6KR7

Crystal structure of methylated human leucyl-tRNA synthetase, Leu-AMS-bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	273
Detector technology	CCD
Collection date	2016-10-09
Detector	MAR CCD 130 mm
Wavelength(s)	1.0000
Spacegroup name	P 65 2 2
Unit cell lengths	136.818, 136.818, 437.266
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.877 - 4.000
R-factor	0.2366
Rwork	0.232
R-free	0.27760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wz2
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	4.070
High resolution limit [Å]	4.000	10.820	4.000
Rmerge	0.106	0.064	0.308
Rmeas	0.112	0.067	0.348
Rpim	0.034	0.019	0.153
Total number of observations	145569
Number of reflections	20622	1237	976
<I/σ(I)>	6.7
Completeness [%]	95.3	97.6	93.9
Redundancy	7.1	11.5	4
CC(1/2)		0.998	0.551

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M HEPES (pH 7.1), 0.42 M Ammonium sulfate, 24 % PEG3350