6KQY

Crystal structure of human leucyl-tRNA synthetase, Leucine-bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	273
Detector technology	CCD
Collection date	2018-03-25
Detector	MAR CCD 165 mm
Wavelength(s)	1.0000
Spacegroup name	P 65 2 2
Unit cell lengths	123.744, 123.744, 536.440
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.760 - 3.300
R-factor	0.2516
Rwork	0.251
R-free	0.27560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wz2
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.850
High resolution limit [Å]	2.800	7.590	2.800
Rmerge	0.189	0.087	0.305
Rmeas	0.200	0.089	0.431
Rpim	0.060	0.017	0.305
Number of reflections	67806	3463	69
<I/σ(I)>	6.2
Completeness [%]	93.5	98.4	2.3
Redundancy	9.1	22.4	1.1
CC(1/2)		0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M HEPES (pH 7.1), 0.42 M Ammonium sulfate, 24 % PEG3350