6KQD
Thermus thermophilus initial transcription complex comprising sigma A and 5'-OH RNA of 3 nt
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-09 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 185.501, 104.044, 297.441 |
| Unit cell angles | 90.00, 98.50, 90.00 |
Refinement procedure
| Resolution | 44.351 - 3.300 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.24990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g7h |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.000 | 45.000 | 3.360 |
| High resolution limit [Å] | 3.300 | 8.940 | 3.300 |
| Rmerge | 0.092 | 0.032 | 0.510 |
| Total number of observations | 433949 | ||
| Number of reflections | 155222 | 7667 | 7932 |
| <I/σ(I)> | 7.1 | ||
| Completeness [%] | 91.6 | 87 | 93.6 |
| Redundancy | 2.8 | 2.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.05 M Magnesium chloride, 0.2 M Potassium chloride, 0.1 M Tris-HCl pH 8.0, 10% PEG 4000 |
