6KPP
BNC105 in complex with tubulin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.455, 157.664, 183.135 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.266 - 2.745 |
R-factor | 0.196346564371 |
Rwork | 0.194 |
R-free | 0.23479 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4i55 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.738 |
Data reduction software | HKL-3000 |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.740 | 2.750 |
Number of reflections | 79756 | 7408 |
<I/σ(I)> | 20.3 | |
Completeness [%] | 98.7 | 93.5 |
Redundancy | 6.6 | |
CC(1/2) | 0.989 | 0.715 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 277 | 6% PEG, 5% glycerol, 0.1M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7 |