6KND

The C-terminal Domain of Translation Initiation Factor 5 at low pH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-01-18
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.1
Spacegroup name	I 2 2 2
Unit cell lengths	48.494, 68.649, 126.815
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.608 - 1.900
R-factor	0.2165
Rwork	0.214
R-free	0.25710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.806
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.608	39.608	2.010
High resolution limit [Å]	1.900	5.650	1.900
Rmerge	0.045	0.016	0.652
Rmeas	0.051	0.018	0.742
Total number of observations	73362
Number of reflections	17005	726	2581
<I/σ(I)>	19.24	59.27	2.37
Completeness [%]	98.8	99.2	94.1
Redundancy	4.314	4.077	4.307
CC(1/2)	0.999	1.000	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	sodium chloride, phosphate-citrate buffer (pH 4.2), 50% (v/v) PEG 200