6KN5

Crystal structure of AFF4 C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2018-09-30
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97845
Spacegroup name	I 21 21 21
Unit cell lengths	41.062, 71.117, 187.417
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.935 - 2.200
R-factor	0.2518
Rwork	0.247
R-free	0.29230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.719
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.940	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.069	0.946
Rmeas	0.072
Rpim	0.020
Number of reflections	14447	1249
<I/σ(I)>	21.8
Completeness [%]	100.0
Redundancy	12.8
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	sodium dihydrogen phosphate, potassium monohydrogen phosphate, 2-(N-morpholino)ethanesulfonic acid