6KMT
P32 of caspase-11 mutant C254A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL19U1 |
Synchrotron site | NFPSS |
Beamline | BL19U1 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2017-11-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97855 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 157.509, 86.469, 93.229 |
Unit cell angles | 90.00, 123.15, 90.00 |
Refinement procedure
Resolution | 46.550 - 2.600 |
R-factor | 0.2053 |
Rwork | 0.202 |
R-free | 0.25380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ice |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.550 | 2.670 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.082 | 0.630 |
Number of reflections | 32377 | 4668 |
<I/σ(I)> | 9.66 | 2.27 |
Completeness [%] | 99.1 | |
Redundancy | 3.4 | |
CC(1/2) | 0.995 | 0.839 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM HEPES (pH 7.5), 26% (w/v) polyethylene glycol 3350, and 180 mM Lithium acetate |