6KMH
The crystal structure of CASK/Mint1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-22 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.978, 96.321, 97.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.232 - 2.400 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.22410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tac |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.231 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 5.060 | 2.350 |
| Rmerge | 0.121 | 0.071 | 0.518 |
| Rmeas | 0.127 | 0.075 | 0.545 |
| Rpim | 0.040 | 0.024 | 0.169 |
| Total number of observations | 381609 | ||
| Number of reflections | 37889 | 3990 | 3725 |
| <I/σ(I)> | 12.1 | ||
| Completeness [%] | 99.9 | 99.3 | 100 |
| Redundancy | 10.1 | 9.6 | 10.2 |
| CC(1/2) | 0.997 | 0.972 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2M KI, 20% PEG 3350, pH 7.0 |
