6KMD
Crystal structure of SeMet-phytochromobilin synthase from tomato in complex with biliverdin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-13 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.975 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 63.925, 63.925, 158.426 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.476 - 2.200 |
| Rwork | 0.177 |
| R-free | 0.21150 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14-3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.770 | 2.330 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 31770 | 5053 |
| <I/σ(I)> | 13.21 | 2.88 |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 6.97 | 6.8 |
| CC(1/2) | 0.997 | 0.864 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | PEG 8000, magnesium chloride, Tris-HCl |
