6KLY

Crystal structure of the type III effector XopAI from Xanthomonas axonopodis pv. citri in space group P43212

Replaces:  4ELN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	110
Detector technology	CCD
Collection date	2010-06-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9762
Spacegroup name	P 43 21 2
Unit cell lengths	73.050, 73.050, 114.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.563 - 2.010
R-factor	0.1671
Rwork	0.166
R-free	0.19490
Structure solution method	MAD
Data reduction software	iMOSFLM (v7.2.2)
Data scaling software	pointless (v1.11.21)
Phasing software	PHENIX (1.14_3260: ???)
Refinement software	PHENIX (1.14_3260: ???)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.350	2.060
High resolution limit [Å]	2.010	2.010
Rmerge	0.082	0.525
Rmeas	0.094	0.605
Rpim	0.043	0.290
Number of reflections	19639	1275
<I/σ(I)>	11.1
Completeness [%]	93.7	85.3
Redundancy	4.1	3.7
CC(1/2)	0.997	0.712

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	15% (v/v) PEG 400, 5.5% (w/v) PEG 20000, 0.05 M Potassium phosphate, pH 8.0
1	VAPOR DIFFUSION, HANGING DROP	8	298	15% (v/v) PEG 400, 5.5% (w/v) PEG 20000, 0.05 M Potassium phosphate, pH 8.0