6KL7
Beta-arrestin 1 mutant S13D/T275D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-25 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.441, 76.835, 119.456 |
| Unit cell angles | 90.00, 100.93, 90.00 |
Refinement procedure
| Resolution | 39.096 - 2.794 |
| R-factor | 0.2211 |
| Rwork | 0.218 |
| R-free | 0.27350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wtr |
| Data reduction software | HKL-2000 (718) |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.8.0) |
| Refinement software | PHENIX (1.16-3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.900 |
| High resolution limit [Å] | 2.794 | 2.800 |
| Rmerge | 0.073 | 0.552 |
| Rmeas | 0.084 | 0.634 |
| Rpim | 0.042 | 0.309 |
| Number of reflections | 27394 | 2715 |
| <I/σ(I)> | 12.89 | 2.3 |
| Completeness [%] | 98.7 | 98.15 |
| Redundancy | 4 | 4.1 |
| CC(1/2) | 0.998 | 0.887 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.05 M HEPES 7.5, 70 mM BaCl2, 9% PEG 3550 |
