6KL4
Crystal structure of MavC-UBE2N-Ub
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97894 |
Spacegroup name | P 65 |
Unit cell lengths | 149.561, 149.561, 58.774 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.000 - 2.850 |
R-factor | 0.1887 |
Rwork | 0.185 |
R-free | 0.26180 |
Structure solution method | SAD |
Data scaling software | xia2 |
Phasing software | HKL-3000 |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.000 | 2.900 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.103 | 1.275 |
Number of reflections | 17794 | 882 |
<I/σ(I)> | 35.5 | 2.6 |
Completeness [%] | 99.9 | |
Redundancy | 20 | |
CC(1/2) | 1.000 | 0.940 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | COUNTER-DIFFUSION | 289.15 | 0.2M Sodium malonate pH 6.0, 20%w/v PEG 3,350 |