6KKZ
Crystal structure of the S65T/F99S/M153T/V163A variant of perdeuterated GFP at pD 8.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.70 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.762, 62.112, 68.911 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.137 - 0.900 |
R-factor | 0.108 |
Rwork | 0.107 |
R-free | 0.12350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wur |
RMSD bond length | 0.014 |
RMSD bond angle | 2.442 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 0.920 |
High resolution limit [Å] | 0.900 | 0.900 |
Rmerge | 0.094 | 0.839 |
Number of reflections | 159331 | 6457 |
<I/σ(I)> | 18 | 1 |
Completeness [%] | 98.3 | 80.3 |
Redundancy | 5.8 | 3 |
CC(1/2) | 0.473 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.1 | 308 | PEG 4000, MgCl2, Tris-DCl buffer |