6KJG
Crystal structure of PsoF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-11 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 189.238, 44.102, 142.616 |
| Unit cell angles | 90.00, 93.59, 90.00 |
Refinement procedure
| Resolution | 19.800 - 1.990 |
| R-factor | 0.1982 |
| Rwork | 0.197 |
| R-free | 0.22960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5thy |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.090 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.054 | 0.332 |
| Number of reflections | 80787 | 12540 |
| <I/σ(I)> | 11.87 | 2.48 |
| Completeness [%] | 98.3 | 95.8 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3350, sodium fluoride, bis-tris propane pH 6.5 |
