6KI2

The STAS domain of cyanobacteria bicarbonate transporter BicA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-02
Detector	DECTRIS PILATUS3 R CdTe 300K
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	57.050, 62.109, 57.036
Unit cell angles	90.00, 119.86, 90.00

Refinement procedure

Resolution	28.581 - 2.197
Rwork	0.213
R-free	0.23010
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.013
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.280
High resolution limit [Å]	2.197	4.730	2.200
Rmerge	0.103	0.083	0.354
Rmeas	0.113	0.091	0.389
Rpim	0.046	0.038	0.159
Number of reflections	34281	1822	1757
<I/σ(I)>	6.2
Completeness [%]	99.8	99.7	99.9
Redundancy	6.1	6.1	5.8
CC(1/2)		0.978	0.938

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20 mM Magnesium chloride, 2 mM Cobalt chloride, 50 mM HEPES, pH7.5, 2.0 M Ammonium sulphate, 1 mM spermine.