6KHZ
p62/SQSTM1 ZZ domain with Gly-peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 173 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-04-14 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 113.979, 113.979, 113.979 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.300 - 2.800 |
| R-factor | 0.24 |
| Rwork | 0.235 |
| R-free | 0.28730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yp7 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.130 | 0.860 |
| Rpim | 0.054 | |
| Number of reflections | 6200 | 6200 |
| <I/σ(I)> | 19.8 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.17 M Ammonium sulfate, 0.085 M Sodium cacodylate trihydrate pH 6.5, 22-30 % w/v Polyethylene glycol 8000 |
