6KHR
Structure of glycinamide-RNase-transformylase T from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 103.132, 103.132, 148.692 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.719 - 2.786 |
| R-factor | 0.2179 |
| Rwork | 0.217 |
| R-free | 0.24220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eyz |
| Data reduction software | MOSFLM |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14rc3_3206) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.719 | 2.940 |
| High resolution limit [Å] | 2.786 | 2.790 |
| Rmerge | 0.266 | 0.874 |
| Rpim | 0.098 | 0.320 |
| Number of reflections | 23300 | 27385 |
| <I/σ(I)> | 8 | 4.7 |
| Completeness [%] | 99.4 | 97 |
| Redundancy | 8.9 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.06 M sodium acetate, pH4.6, 6.0% PEG4000, 32% glycerol. |
