6KFP

Crystal structure of MavC ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	97.704, 105.860, 125.391
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.530 - 2.920
R-factor	0.196
Rwork	0.193
R-free	0.24980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tsc 1ubq
RMSD bond length	0.003
RMSD bond angle	0.606
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER (1.15_3459)
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.020
High resolution limit [Å]	2.920	2.920
Rmerge	0.098	0.794
Number of reflections	14312	1390
<I/σ(I)>	26.6
Completeness [%]	99.4
Redundancy	12
CC(1/2)	0.997	0.882

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, lithium chloride