6KF8
OsACBP2 in complex with C18:3-CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-11-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97894 |
| Spacegroup name | H 3 |
| Unit cell lengths | 149.965, 149.965, 95.443 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.830 - 3.600 |
| R-factor | 0.2299 |
| Rwork | 0.228 |
| R-free | 0.27920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5h3i |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.411 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.910 | 3.700 |
| High resolution limit [Å] | 3.510 | 3.510 |
| Rmerge | 0.136 | 0.522 |
| Number of reflections | 9948 | 1445 |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 99.5 | |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M MES pH 6.5, 30% PEG 4000 |
