6KE2

ABloop reengineered Ferritin Nanocage

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9779
Spacegroup name	F 4 3 2
Unit cell lengths	182.448, 182.448, 182.448
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.334 - 1.798
R-factor	0.1565
Rwork	0.154
R-free	0.17940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fha
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.798	1.800
Rmerge	0.073	0.200
Number of reflections	24788	1207
<I/σ(I)>	53.8
Completeness [%]	99.9
Redundancy	21.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		285	0.1M bincine pH 9.0, 2M MgCl2