6KE1
Crystal structure of TtCas1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97907 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 150.432, 150.432, 219.430 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.600 - 3.390 |
R-factor | 0.2212 |
Rwork | 0.221 |
R-free | 0.22930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6kdv |
RMSD bond length | 0.004 |
RMSD bond angle | 1.016 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.460 |
High resolution limit [Å] | 3.390 | 3.400 |
Number of reflections | 19271 | 953 |
<I/σ(I)> | 28.8 | |
Completeness [%] | 99.4 | 99.7 |
Redundancy | 8.6 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 1.8-2.3M NaCl, 0.1M Bis-Tris pH 5.5 |