6KD2

Room temperature structure of glucose isomerase delivered in gelatin by serial millisecond crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	298.15
Detector technology	PIXEL
Collection date	2019-04-03
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.9796
Spacegroup name	I 2 2 2
Unit cell lengths	94.520, 100.170, 103.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.003 - 1.700
R-factor	0.1931
Rwork	0.193
R-free	0.22360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zye
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.400	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	54280	5364
<I/σ(I)>	5.15	1.73
Completeness [%]	100.0	100
Redundancy	1488.7	1021.5
CC(1/2)	0.982	0.446

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7	293.5	Tris-HCl, Ammonium sulfate, Magnesium sulfate