6KBM

Crystal structure of Vac8 bound to Atg13

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	62.727, 92.391, 139.879
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.841 - 2.902
R-factor	0.208
Rwork	0.206
R-free	0.24590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xjg
RMSD bond length	0.004
RMSD bond angle	0.824
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.900	2.900
Rmerge	0.120	0.435
Number of reflections	17745	794
<I/σ(I)>	11.27
Completeness [%]	95.2
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 400, Tris, Ethylene glycol, PEG 3350, DTT