6KBI
Crystal structure of ErbB3 N418Q mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 236.105, 49.458, 190.919 |
| Unit cell angles | 90.00, 125.62, 90.00 |
Refinement procedure
| Resolution | 46.623 - 3.000 |
| R-factor | 0.224 |
| Rwork | 0.221 |
| R-free | 0.27790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m6b |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.700 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmeas | 0.105 | 0.564 |
| Number of reflections | 36623 | 5774 |
| <I/σ(I)> | 11.4 | 2.2 |
| Completeness [%] | 99.1 | 97.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2 M lithium sulfate, 0.1 M Tris buffer (pH 8.5) and 11% (w/v) PEG4000. |
