6K9N
Rice_OTUB_like_catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9873 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.688, 144.810, 155.848 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.380 - 2.270 |
| R-factor | 0.1879 |
| Rwork | 0.186 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zfy |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.717 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.290 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rpim | 0.035 | 0.158 |
| Number of reflections | 62083 | 3018 |
| <I/σ(I)> | 7.54 | |
| Completeness [%] | 99.7 | |
| Redundancy | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 291 | 0.15 M Potassium bromide, 30% w/v Polyethylene glycol monomethyl ether 2,000 |
