6K9D
glycoside hydrolase family 12 (GH12) englucanase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.00919 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 88.631, 88.631, 123.411 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.879 - 1.505 |
R-factor | 0.1738 |
Rwork | 0.173 |
R-free | 0.18620 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.806 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.879 | 4.279 |
High resolution limit [Å] | 1.505 | 1.505 |
Number of reflections | 62640 | 23 |
<I/σ(I)> | 1.34 | |
Completeness [%] | 80.0 | |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | NfEG12A-N18Y was concentrated to 10 mg/ml in a buffer containing 100 mM citric acid-Na2HPO4 (pH 7.2). both proteins were crystallized in a mother liquid with 0.1 M citrate (pH 5.0) and 16% (w/v) PEG 20000 using the hanging drop vapor diffusion method in 24-well plates. NfEG12A-N18Y crystals was transferred to a reservoir solution supplemented with 25% (v/v) ethylene glycol to grown. Obtained crystals were cryoprotected with 25% (v/v) 2-methyl-2,4-pentanediol. The crystals were mounted on a nylon loop and cooled immediately in liquid nitrogen prior to data collection. |