6K9B
Apo structure of NrS-1 N terminal domain N305
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-03-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.136, 107.555, 66.868 |
| Unit cell angles | 90.00, 113.34, 90.00 |
Refinement procedure
| Resolution | 29.520 - 2.450 |
| R-factor | 0.19025 |
| Rwork | 0.188 |
| R-free | 0.23167 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.163 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.120 | 0.375 |
| Rmeas | 0.134 | 0.438 |
| Number of reflections | 24723 | 2338 |
| <I/σ(I)> | 10.2 | 2.4 |
| Completeness [%] | 97.1 | 92.4 |
| Redundancy | 4.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291.15 | 0.1M Bis-tris pH 6.5, 25% w/v PEG 3350 |
