6K95
Crystal structural of human glutathione-specific gamma-glutamylcyclotransferase 2 (ChaC2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.90000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.842, 62.161, 103.625 |
| Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
| Resolution | 37.410 - 2.290 |
| R-factor | 0.2405 |
| Rwork | 0.239 |
| R-free | 0.26840 |
| Structure solution method | MAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.288 | 2.300 |
| Number of reflections | 31131 | |
| <I/σ(I)> | 23.006 | |
| Completeness [%] | 99.5 | |
| Redundancy | 5.3 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 14%(w/v) polyethylene-glycol 8,000 (PEG 8000), 14% PEG, 100mM HEPES (pH 7.5) |
